Growth adaptability and foreign gene stability of TaLEA transgenic Populus simonii × nigra

Key message

Late-embryogenesis-abundant proteins (LEA) typically accumulate to high levels during seed dehydration and have an important role in the plant response to abiotic stresses. Transgenic hybrid poplars of 9-year-old TaLEA lines showed adaptation to drought and saline–alkali conditions. The exogenous gene was expressed normally, and the T-DNA insertion sites were identified on different chromosomes.


Context Improving the survival rate of afforested species is essential for forest production in saline–alkali areas. Transgenic hybrid poplars could provide potential genetic materials for adaptation to semi-arid areas.
Aims We investigated the growth stability and adaptability of 9-year-old TaLEA transgenic lines of Populus simonii × nigra, as well as the position of T-DNA insertion and the stability of exogenous genes.
Methods Transgenic poplar field trials were carried out using a multi-site joint analysis. Molecular characteristics and the T-DNA insertion sites were identified by genome resequencing.
Results The XL-9, XL-7, and XL-13 preferred lines consistently showed the highest and most stable growth across three testing sites. The exogenous gene was integrated into the genome chromosome and was expressed normally in the XL-1, XL-7, and XL-9 lines after 9 years.
Conclusion XL-9, XL-7, and XL-13 are the preferred lines for subsequent production tests. Transgenic hybrid poplar lines can be selected for adaptability 2 years after field experiments. The results of this study will provide further guidance for testing of transgenic trees for future forest production.

Populus simonii × nigra; Late-embryogenesis-abundant proteins; Abiotic stress; Field experiment

Wang, Y., Yang, Y., Wang, F. et al. Growth adaptability and foreign gene stability of TaLEA transgenic Populus simonii × nigra. Annals of Forest Science 78, 42 (2021).

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Data availability
The datasets generated and/or analyzed during the current study are available in the Science Data Bank repository,

Handling Editor
Ricardo Alia

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