Genetic variation in susceptibility to pine wilt disease of maritime pine (Pinus pinaster Aiton) half-sib families

Topical collection: Mediterranean Pines

This paper presents a greenhouse study for assessing the genetic variation in maritime pine (Pinus pinaster Aiton) in response to pinewood nematode (Bursaphelenchus xylophilus (Steiner et Buhren) Nickle), which is a causal agent of pine wilt disease. Fifteen out of 96 half-sib families were selected as less susceptible. This experiment is an important first step for creating a resistance breeding program.

Context Pine wilt disease is caused by the pinewood nematode (Bursaphelenchus xylophilus (Steiner et Buhren) Nickle), a quarantine pest, and is a concern to maritime pine (Pinus pinaster) in Portugal due to its economic, environmental, and social impacts. This disease is regarded as a major threat to European forests.
Aims This paper aimed to evaluate the genetic variation in maritime pine families that were inoculated with pinewood nematode, identify the most resistant families, and establish the guidelines for a resistance improvement program.
Methods Two-year-old half-sib progenies obtained from 96 plus trees were inoculated. The plants were monitored for survival on four different dates. The statistical analysis followed the mixed model theory.
Results Genetic variability of the susceptibility to pine wilt disease was observed. At 157 days after inoculation, the 15 highest genetic ranking families out of 96 were selected, having a predicted survival mean of 15.6% instead of 11.0% on average for the all 96 families.
Conclusion This study allows for the implementation of an improvement program to help control pine wilt disease.

Genetic variability, Mixed models, Bursaphelenchus xylophilus, Progeny test, Tree improvement, Resistance

Carrasquinho, I., Lisboa, A., Inácio, M.L. et al. Annals of Forest Science (2018) 75: 85.

For the read-only version of the full text:

Data availability
The phenotypic data have been deposited at in PANGAEA repository.
Carrasquinho I, Lisboa A, Inácio ML, Gonçalves E (2018) Observed genetic variation to pine wood nematode in maritime pine two years old half-sibs plants. PANGAEA. [Dataset].

Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.