
Nitrification and nitrogen (N) immobilization are important pathways in soil N transformations, involving soil N loss and retention, respectively. The ratio of nitrification to N immobilization generally reflects the potential risk of soil N loss. However, little is known about the response of this ratio to anthropogenic carbon (C) and N inputs, but also climate and soil conditions. Here, we aimed to elucidate, for the first time, the impacts of chemical fertilizer and manure application on the ratio of gross nitrification to N immobilization by using 15N dilution technology, based on ten long-term fertilization trials spanning multiple climatic zones in eastern China. Results showed that manure application differentially increased gross N immobilization rather than nitrification compared to the chemical fertilizer treatment, leading to manure-induced decreases in gross nitrification to N immobilization ratio ranging from 1.2 to 93% across the sites. The decreased gross nitrification to N immobilization ratio in the manure treatment was mainly due to the increased ratio of bacteria to nitrifiers abundance. Manuring was more effective for a decrease in the gross nitrification to N immobilization ratio at sites characterized by high rainfall and low soil pH, as it prevented soil pH decline thereby favoring bacterial abundance and N immobilization. Consequently, manure application resulted in a substantial increase in soil total N accumulation, facilitated by increased microbial N immobilization that promoted microbial biomass. These findings suggest that substituting manure for chemical fertilizer in the areas with high rainfall and acidic soils promisingly reduces soil N loss risk, with positive consequences for soil N retention. This knowledge highlights the potential to reconcile soil N loss and fertility improvement through optimizing regional manure management, which offers valuable insights for the development of a tailored regional fertilization management strategy.
Wang, J., Zhang, L., Liu, K. et al. Minimizing the potential risk of soil nitrogen loss through optimal fertilization practices in intensive agroecosystems. Agron. Sustain. Dev. 45, 9 (2025). https://doi.org/10.1007/s13593-025-01006-5