{"id":4882,"date":"2021-04-16T08:02:17","date_gmt":"2021-04-16T06:02:17","guid":{"rendered":"https:\/\/ist.blogs.inrae.fr\/afs\/?p=4882"},"modified":"2021-04-15T16:03:41","modified_gmt":"2021-04-15T14:03:41","slug":"growth-adaptability-and-foreign-gene-stability-of-talea-transgenic-populus-simonii-x-nigra","status":"publish","type":"post","link":"https:\/\/ist.blogs.inrae.fr\/afs\/2021\/04\/16\/growth-adaptability-and-foreign-gene-stability-of-talea-transgenic-populus-simonii-x-nigra\/","title":{"rendered":"Growth adaptability and foreign gene stability of TaLEA transgenic Populus simonii \u00d7 nigra"},"content":{"rendered":"<script type='text\/javascript' src='https:\/\/d1bxh8uas1mnw7.cloudfront.net\/assets\/embed.js'><\/script><p><strong><a href=\"https:\/\/ist.blogs.inrae.fr\/afs\/wp-content\/uploads\/sites\/5\/2021\/04\/Wang-Y.-2021.png\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-4886 alignright\" src=\"https:\/\/ist.blogs.inrae.fr\/afs\/wp-content\/uploads\/sites\/5\/2021\/04\/Wang-Y.-2021-300x249.png\" alt=\"\" width=\"300\" height=\"249\" srcset=\"https:\/\/ist.blogs.inrae.fr\/afs\/wp-content\/uploads\/sites\/5\/2021\/04\/Wang-Y.-2021-300x249.png 300w, https:\/\/ist.blogs.inrae.fr\/afs\/wp-content\/uploads\/sites\/5\/2021\/04\/Wang-Y.-2021-768x638.png 768w, https:\/\/ist.blogs.inrae.fr\/afs\/wp-content\/uploads\/sites\/5\/2021\/04\/Wang-Y.-2021-640x531.png 640w, https:\/\/ist.blogs.inrae.fr\/afs\/wp-content\/uploads\/sites\/5\/2021\/04\/Wang-Y.-2021.png 831w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a>Key message<\/strong><\/p>\n<p align=\"justify\">Late-embryogenesis-abundant proteins (LEA) typically accumulate to high levels during seed dehydration and have an important role in the plant response to abiotic stresses. Transgenic hybrid poplars of 9-year-old <em>TaLEA<\/em> lines showed adaptation to drought and saline\u2013alkali conditions. The exogenous gene was expressed normally, and the T-DNA insertion sites were identified on different chromosomes.<\/p>\n<p><strong>Abstract<\/strong><\/p>\n<p align=\"justify\"><strong>Context<\/strong> Improving the survival rate of afforested species is essential for forest production in saline\u2013alkali areas. Transgenic hybrid poplars could provide potential genetic materials for adaptation to semi-arid areas.<br \/>\n<strong>Aims<\/strong> We investigated the growth stability and adaptability of 9-year-old <em>TaLEA<\/em> transgenic lines of <em>Populus simonii<\/em> \u00d7 <em>nigra<\/em>, as well as the position of T-DNA insertion and the stability of exogenous genes.<br \/>\n<strong>Methods<\/strong> Transgenic poplar field trials were carried out using a multi-site joint analysis. Molecular characteristics and the T-DNA insertion sites were identified by genome resequencing.<br \/>\n<strong>Results<\/strong> The XL-9, XL-7, and XL-13 preferred lines consistently showed the highest and most stable growth across three testing sites. The exogenous gene was integrated into the genome chromosome and was expressed normally in the XL-1, XL-7, and XL-9 lines after 9 years.<br \/>\n<strong>Conclusion<\/strong> XL-9, XL-7, and XL-13 are the preferred lines for subsequent production tests. Transgenic hybrid poplar lines can be selected for adaptability 2 years after field experiments. The results of this study will provide further guidance for testing of transgenic trees for future forest production.<\/p>\n<p><strong>Keywords<\/strong><br \/>\n<em>Populus simonii<\/em> \u00d7 <em>nigra<\/em>; Late-embryogenesis-abundant proteins; Abiotic stress; Field experiment<\/p>\n<div class='altmetric-embed' data-badge-type='donut' data-doi='10.1007\/s13595-021-01038-3'  style='float: right; ' ><\/div>\n<p><strong>Publication<\/strong><br \/>\nWang, Y., Yang, Y., Wang, F. et al. Growth adaptability and foreign gene stability of <em>TaLEA<\/em> transgenic <em>Populus simonii<\/em> \u00d7 <em>nigra<\/em>. Annals of Forest Science 78, 42 (2021). <a href=\"https:\/\/doi.org\/10.1007\/s13595-021-01038-3\">https:\/\/doi.org\/10.1007\/s13595-021-01038-3<\/a><\/p>\n<p><strong>For the read-only version of the full text:<\/strong><br \/>\n<a href=\"https:\/\/rdcu.be\/ciKAE\">https:\/\/rdcu.be\/ciKAE<\/a><\/p>\n<p><strong>Data availability<\/strong><br \/>\nThe datasets generated and\/or analyzed during the current study are available in the Science Data Bank repository, <a href=\"https:\/\/doi.org\/10.11922\/sciencedb.00435\">https:\/\/doi.org\/10.11922\/sciencedb.00435<\/a><\/p>\n<p><strong>Handling Editor<\/strong><br \/>\nRicardo Alia<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key message Late-embryogenesis-abundant proteins (LEA) typically accumulate to high levels during seed dehydration and have an important role in the plant response to abiotic stresses. Transgenic hybrid poplars of 9-year-old TaLEA lines showed adaptation to drought and saline\u2013alkali conditions. The exogenous gene was expressed normally, and the T-DNA insertion sites were identified on different chromosomes. [&hellip;]<\/p>\n","protected":false},"author":109,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[14,110,15],"tags":[],"class_list":["post-4882","post","type-post","status-publish","format-standard","hentry","category-article-type","category-data-in-repository","category-research-paper","cat-14-id","cat-110-id","cat-15-id"],"_links":{"self":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/posts\/4882","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/users\/109"}],"replies":[{"embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/comments?post=4882"}],"version-history":[{"count":0,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/posts\/4882\/revisions"}],"wp:attachment":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/media?parent=4882"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/categories?post=4882"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/tags?post=4882"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}