{"id":3986,"date":"2020-02-25T09:00:20","date_gmt":"2020-02-25T08:00:20","guid":{"rendered":"https:\/\/ist.blogs.inrae.fr\/afs\/?p=3986"},"modified":"2020-02-19T15:52:58","modified_gmt":"2020-02-19T14:52:58","slug":"clinal-variation-along-precipitation-gradients-in-patagonian-temperate-forests-unravelling-demographic-and-selection-signatures-in-three-nothofagus-spp","status":"publish","type":"post","link":"https:\/\/ist.blogs.inrae.fr\/afs\/2020\/02\/25\/clinal-variation-along-precipitation-gradients-in-patagonian-temperate-forests-unravelling-demographic-and-selection-signatures-in-three-nothofagus-spp\/","title":{"rendered":"Clinal variation along precipitation gradients in Patagonian temperate forests: unravelling demographic and selection signatures in three Nothofagus spp."},"content":{"rendered":"<script type='text\/javascript' src='https:\/\/d1bxh8uas1mnw7.cloudfront.net\/assets\/embed.js'><\/script><div id=\"attachment_3989\" style=\"width: 310px\" class=\"wp-caption alignleft\"><a href=\"https:\/\/ist.blogs.inrae.fr\/afs\/wp-content\/uploads\/sites\/5\/2020\/02\/Soliani-et-al.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-3989\" class=\"size-medium wp-image-3989\" src=\"https:\/\/ist.blogs.inrae.fr\/afs\/wp-content\/uploads\/sites\/5\/2020\/02\/Soliani-et-al-300x256.png\" alt=\"\" width=\"300\" height=\"256\" srcset=\"https:\/\/ist.blogs.inrae.fr\/afs\/wp-content\/uploads\/sites\/5\/2020\/02\/Soliani-et-al-300x256.png 300w, https:\/\/ist.blogs.inrae.fr\/afs\/wp-content\/uploads\/sites\/5\/2020\/02\/Soliani-et-al-1024x872.png 1024w, https:\/\/ist.blogs.inrae.fr\/afs\/wp-content\/uploads\/sites\/5\/2020\/02\/Soliani-et-al-768x654.png 768w, https:\/\/ist.blogs.inrae.fr\/afs\/wp-content\/uploads\/sites\/5\/2020\/02\/Soliani-et-al-640x545.png 640w, https:\/\/ist.blogs.inrae.fr\/afs\/wp-content\/uploads\/sites\/5\/2020\/02\/Soliani-et-al.png 1141w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><p id=\"caption-attachment-3989\" class=\"wp-caption-text\">Sampled locations of N. nervosa in the L\u00e1car Lake watershed<\/p><\/div>\n<p align=\"justify\"><strong>Key message<\/strong> Past demographic changes and current selection pressures determine the genetic variation displayed by <em>Nothofagus<\/em> species along rainfall gradients. Based on the diversity trends observed at candidate genes associated to drought stress, we inferred a differential species\u2019 adaptive potential.<br \/>\n<strong>Context<\/strong> Clinal genetic variation in natural populations could reflect either recent demographic history or the evolution of adapted genotypes along heterogeneous environments.<br \/>\n<strong>Aims<\/strong> We describe genetic variation patterns in three <em>Nothofagus<\/em> species of South American temperate forests, growing along steep rainfall gradients. Our hypothesis is that the selection pressure along this gradient reinforces the genetic structure previously shaped by Pleistocene climate oscillations.<br \/>\n<strong>Methods<\/strong> We screened variation along gradients at putative adaptive markers: candidate genes involved in response to drought, and EST-SSRs linked to drought stress genes. Genomic SSRs (gSSRs) were used to decouple the incidence of demographic events in the genetic structure.<br \/>\n<strong>Results<\/strong> Genetic diversity at SSRs agreed with the putative location of cryptic Pleistocene refugia in <em>Nothofagus<\/em>. In addition, each species showed different trends for nucleotide diversity at candidate genes. Unbiased heterozygosity significantly correlated with precipitation at EST-SSRs in <em>Nothofagus nervosa<\/em>. We found evidences of balancing selection and several SNPs departed from neutral expectations.<br \/>\n<strong>Conclusions<\/strong> <em>Nothofagus<\/em> genetic variability shows a strong imprint of demographic changes that reveals refugia location for the species during Pleistocene. This variability is modelled by environmental conditions across natural gradients, which impose selection pressure at genome regions related to stress response, providing clues about inter-specific differences in adaptive potential to water deficit.<\/p>\n<p><strong>Keywords<\/strong><br \/>\nRainfall gradient, Genetic diversity, Candidate genes, Drought stress, <em>Nothofagus<\/em>, Evolutionary history<\/p>\n<div class='altmetric-embed' data-badge-type='donut' data-doi='10.1007\/s13595-019-0908-x'  style='float: right; ' ><\/div>\n<p><strong>Publication<\/strong><br \/>\nSoliani, C., Azpilicueta, M.M., Arana, M.V. et al. Clinal variation along precipitation gradients in Patagonian temperate forests: unravelling demographic and selection signatures in three Nothofagus spp.. Annals of Forest Science 77, 4 (2020). <a href=\"https:\/\/doi.org\/10.1007\/s13595-019-0908-x\">https:\/\/doi.org\/10.1007\/s13595-019-0908-x<\/a><\/p>\n<p>For the read-only version of the full text: <a href=\"https:\/\/rdcu.be\/b1UMw\">https:\/\/rdcu.be\/b1UMw<\/a><\/p>\n<p align=\"justify\"><strong>Data availability<\/strong><br \/>\nIndividual gene sequences were deposited to GeneBank (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/\">https:\/\/www.ncbi.nlm.nih.gov\/<\/a>). Accession numbers MF446356-MF446390; Multilocus SSRs genotypes, candidate genes genotypes and the supplementary material were deposited in Zenodo repository (Soliani et al. 2019): <a href=\"https:\/\/doi.org\/10.5281\/zenodo.3544568\">https:\/\/doi.org\/10.5281\/zenodo.3544568<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Key message Past demographic changes and current selection pressures determine the genetic variation displayed by Nothofagus species along rainfall gradients. Based on the diversity trends observed at candidate genes associated to drought stress, we inferred a differential species\u2019 adaptive potential. Context Clinal genetic variation in natural populations could reflect either recent demographic history or the [&hellip;]<\/p>\n","protected":false},"author":106,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[14,110,15],"tags":[],"class_list":["post-3986","post","type-post","status-publish","format-standard","hentry","category-article-type","category-data-in-repository","category-research-paper","cat-14-id","cat-110-id","cat-15-id"],"_links":{"self":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/posts\/3986","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/users\/106"}],"replies":[{"embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/comments?post=3986"}],"version-history":[{"count":0,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/posts\/3986\/revisions"}],"wp:attachment":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/media?parent=3986"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/categories?post=3986"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/tags?post=3986"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}