{"id":3852,"date":"2019-11-19T09:00:27","date_gmt":"2019-11-19T08:00:27","guid":{"rendered":"https:\/\/ist.blogs.inra.fr\/afs\/?p=3852"},"modified":"2019-11-18T16:25:23","modified_gmt":"2019-11-18T15:25:23","slug":"proteomic-analysis-of-black-poplar-populus-nigra-l-seed-storability","status":"publish","type":"post","link":"https:\/\/ist.blogs.inrae.fr\/afs\/2019\/11\/19\/proteomic-analysis-of-black-poplar-populus-nigra-l-seed-storability\/","title":{"rendered":"Proteomic analysis of black poplar (Populus nigra L.) seed storability"},"content":{"rendered":"<script type='text\/javascript' src='https:\/\/d1bxh8uas1mnw7.cloudfront.net\/assets\/embed.js'><\/script><div id=\"attachment_3854\" style=\"width: 330px\" class=\"wp-caption alignleft\"><a href=\"https:\/\/ist.blogs.inra.fr\/afs\/wp-content\/uploads\/sites\/5\/2019\/11\/Pawlowski-et-al.png\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-3854\" class=\"wp-image-3854\" src=\"https:\/\/ist.blogs.inra.fr\/afs\/wp-content\/uploads\/sites\/5\/2019\/11\/Pawlowski-et-al-300x227.png\" alt=\"\" width=\"320\" height=\"242\" srcset=\"https:\/\/ist.blogs.inrae.fr\/afs\/wp-content\/uploads\/sites\/5\/2019\/11\/Pawlowski-et-al-300x227.png 300w, https:\/\/ist.blogs.inrae.fr\/afs\/wp-content\/uploads\/sites\/5\/2019\/11\/Pawlowski-et-al-768x582.png 768w, https:\/\/ist.blogs.inrae.fr\/afs\/wp-content\/uploads\/sites\/5\/2019\/11\/Pawlowski-et-al-1024x777.png 1024w, https:\/\/ist.blogs.inrae.fr\/afs\/wp-content\/uploads\/sites\/5\/2019\/11\/Pawlowski-et-al-640x485.png 640w, https:\/\/ist.blogs.inrae.fr\/afs\/wp-content\/uploads\/sites\/5\/2019\/11\/Pawlowski-et-al.png 1262w\" sizes=\"auto, (max-width: 320px) 100vw, 320px\" \/><\/a><p id=\"caption-attachment-3854\" class=\"wp-caption-text\">Reference gel presenting positions of statistically variable spots from Populus nigra L. seeds stored at different temperature conditions of 3, \u2212\u20093, \u2212\u200920, and \u2212\u2009196 \u00b0C for 12 and 24 months<\/p><\/div>\n<p align=\"justify\">Adequate storability of black poplar ( <em>Populus nigra<\/em> L.) seeds at temperatures below 0 \u00b0C was associated with preservation of proteins of energy and carbohydrate metabolism, protein turnover, and proteins maintaining long-term stability of dehydrated tissue.<\/p>\n<p align=\"justify\"><strong>Context<\/strong> Understanding seed storability is a key factor for effective seed preservation and conservation. Black poplar is an endangered tree species and its seed loses rapidly viability during storage.<br \/>\n<strong>Aims<\/strong> The aim of this study was to determine, and functionally characterise, the proteins associated with storability of black poplar seeds.<br \/>\n<strong>Methods<\/strong> Dried seeds (7.1% MC) were stored at 3 \u00b0C, \u2212\u20093 \u00b0C, \u2212\u200920 \u00b0C, and \u2212\u2009196 \u00b0C, for a period of 12 and 24 months. Proteins were extracted and separated according to their isoelectric point (pI) and mass using 2-dimensional electrophoresis. Proteins that varied in abundance for temperature and time of storage were identified by mass spectrometry.<br \/>\n<strong>Results<\/strong> A germination test showed that seeds remained viable at \u2212\u20093, \u2212\u200920, and \u2212\u2009196 \u00b0C. Storage at 3 \u00b0C caused loss of seed viability. This loss in seed vigour was related to the largest changes in protein abundance. As storage temperature decreased, a smaller number of proteins displayed changed abundance.<br \/>\n<strong>Conclusion<\/strong> Good storability of black poplar seeds under freezing conditions may be associated with the following: an inhibition of synthesis of energy and carbohydrate metabolism and protein turnover proteins, LEA proteins that maintain long-term stability of dehydrated tissue, GDSL esterases\/lipases that inhibit hydrolysing activity, and by chaperonins that protect protein functionality. Storage conditions influence the preservation of protein function that governs seed viability.<\/p>\n<p><strong>Keywords<\/strong><br \/>\nChilling stress, Germination, Longevity, Proteomics, Seed viability, Seed vigour<\/p>\n<div class='altmetric-embed' data-badge-type='donut' data-doi='10.1007\/s13595-019-0887-y'  style='float: right; ' ><\/div>\n<p><strong>Open-access Publication<\/strong><br \/>\nPaw\u0142owski, T.A., Klupczy\u0144ska, E.A., Staszak, A.M. et al. Annals of Forest Science (2019) 76: 104. <a href=\"https:\/\/doi.org\/10.1007\/s13595-019-0887-y\">https:\/\/doi.org\/10.1007\/s13595-019-0887-y<\/a><\/p>\n<p><strong>Data availability<\/strong><br \/>\nThe datasets generated during and\/or analysed during the current study are available in the Zenodo repository (Paw\u0142owski et al. 2019) at <a href=\"https:\/\/zenodo.org\/record\/3384725#.XW5dC0fgphE\">https:\/\/zenodo.org\/record\/3384725#.XW5dC0fgphE<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Adequate storability of black poplar ( Populus nigra L.) seeds at temperatures below 0 \u00b0C was associated with preservation of proteins of energy and carbohydrate metabolism, protein turnover, and proteins maintaining long-term stability of dehydrated tissue. Context Understanding seed storability is a key factor for effective seed preservation and conservation. Black poplar is an endangered [&hellip;]<\/p>\n","protected":false},"author":106,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[14,110,109,15],"tags":[],"class_list":["post-3852","post","type-post","status-publish","format-standard","hentry","category-article-type","category-data-in-repository","category-open-access","category-research-paper","cat-14-id","cat-110-id","cat-109-id","cat-15-id"],"_links":{"self":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/posts\/3852","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/users\/106"}],"replies":[{"embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/comments?post=3852"}],"version-history":[{"count":0,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/posts\/3852\/revisions"}],"wp:attachment":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/media?parent=3852"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/categories?post=3852"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/tags?post=3852"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}