{"id":3360,"date":"2019-04-04T09:00:14","date_gmt":"2019-04-04T07:00:14","guid":{"rendered":"https:\/\/ist.blogs.inra.fr\/afs\/?p=3360"},"modified":"2019-03-27T13:27:27","modified_gmt":"2019-03-27T12:27:27","slug":"dynamic-species-specific-metabolic-changes-in-the-trees-exposed-to-chronic-ns-additions-at-the-bear-brook-watershed-in-maine-usa","status":"publish","type":"post","link":"https:\/\/ist.blogs.inrae.fr\/afs\/2019\/04\/04\/dynamic-species-specific-metabolic-changes-in-the-trees-exposed-to-chronic-ns-additions-at-the-bear-brook-watershed-in-maine-usa\/","title":{"rendered":"Dynamic species-specific metabolic changes in the trees exposed to chronic N+S additions at the Bear Brook Watershed in Maine, USA"},"content":{"rendered":"<script type='text\/javascript' src='https:\/\/d1bxh8uas1mnw7.cloudfront.net\/assets\/embed.js'><\/script><p align=\"justify\">Red spruce (<em>Picea rubens<\/em> Sarg.) and American beech (<em>Fagus grandifolia<\/em> Ehrh.) nutritional imbalances observed during 1998\u20132000 in response to nitrogen additions beginning in 1989 at Bear Brook Watershed in Maine, USA, were reversed by 2013. However, nitrogen-containing metabolites continued to accumulate to detoxify ammonia. While sugar maple (<em>Acer saccharum<\/em> Marsh.) was N-limited and benefitted from N+S additions, spruce and birch established new homeostatic status via adjusting cellular metabolism.<\/p>\n<p align=\"justify\"><strong>Context<\/strong> Increased deposition of atmospheric N leads to changes in forest productivity. Effects of added N+S on changes in cellular metabolism will yield information on species-specific sensitivity to N+S.<br \/>\n<strong>Aims<\/strong> To evaluate foliar metabolic changes in American beech (<em>Fagus grandifolia<\/em> Ehrh.), sugar maple (<em>Acer saccharum<\/em> Marsh.), and red spruce (<em>Picea rubens<\/em> Sarg.) that were exposed to ammonium sulfate [(NH<sub>4<\/sub>)<sub>2<\/sub>SO<sub>4<\/sub>); ~\u200928.8 kg S ha<sup>\u22121<\/sup> yr<sup>\u22121<\/sup> and 25.2 kg N ha<sup>\u22121<\/sup> yr<sup>\u22121<\/sup>] additions at West Bear Watershed (WBW) starting in 1989 until the end of this experiment, while East Bear Watershed served as a reference.<br \/>\n<strong>Methods<\/strong> Foliage was collected in 1998\u20132000 and 2013. Sapwood plugs were also collected in 2013. All were analyzed for ions and metabolites using HPLC and ICP.<br \/>\n<strong>Results<\/strong> During 1998\u20132000, only N+S-treated beech and spruce foliage had a reduction in Ca and Mg. All species had significantly higher content of N-rich metabolites. In 2013, ammonia detoxification continued in the absence of nutrient deficiencies. Significant changes in growth promoting metabolites occurred only in maple throughout this study.<br \/>\n<strong>Conclusion<\/strong> Metabolic changes indicated that sugar maple at this site was and still is N-limited, whereas red spruce and American beech had to make metabolic adjustments in order to survive under chronic N+S inputs. We conclude that even in the absence of knowledge about individual species tolerance limits for nutrients and critical N load for the site, monitoring with a suite of metabolites that are centrally connected to both C and N pathways could be a very useful tool in assessing stress from nutrient imbalance in various tree species.<\/p>\n<p><strong>Keywords<\/strong><br \/>\nAmerican beech, Bear brook watershed in Maine, Foliar metabolism, Nitrogen plus sulfur addition, Red spruce, Sugar maple<\/p>\n<div class='altmetric-embed' data-badge-type='donut' data-doi='10.1007\/s13595-019-0808-0'  style='float: right; ' ><\/div>\n<p><strong>Publication<\/strong><br \/>\nMinocha, R., Long, S., Turlapati, S.A. et al. Annals of Forest Science (2019) 76: 25. <a href=\"https:\/\/doi.org\/10.1007\/s13595-019-0808-0\">https:\/\/doi.org\/10.1007\/s13595-019-0808-0<\/a><\/p>\n<p>For the read-only version of the full text: <a href=\"https:\/\/rdcu.be\/btiL8\">https:\/\/rdcu.be\/btiL8<\/a><\/p>\n<p align=\"justify\"><strong>Data availability<\/strong><br \/>\nData for this manuscript are available in the Forest Service Research Data Archive (Minocha et al. 2018). Minocha R., Long S., Turlapati S.A., and Fernandez I. (2018) Dynamic species-specific metabolic changes in the trees exposed to chronic N+S additions at the Bear Brook Watershed in Maine, USA. Fort Collins, CO: Forest Service Research Data Archive. V1. [Dataset]. <a href=\"https:\/\/doi.org\/10.2737\/RDS-2018-0058\">https:\/\/doi.org\/10.2737\/RDS-2018-0058<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Red spruce (Picea rubens Sarg.) and American beech (Fagus grandifolia Ehrh.) nutritional imbalances observed during 1998\u20132000 in response to nitrogen additions beginning in 1989 at Bear Brook Watershed in Maine, USA, were reversed by 2013. However, nitrogen-containing metabolites continued to accumulate to detoxify ammonia. While sugar maple (Acer saccharum Marsh.) was N-limited and benefitted from [&hellip;]<\/p>\n","protected":false},"author":106,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[14,110,15],"tags":[],"class_list":["post-3360","post","type-post","status-publish","format-standard","hentry","category-article-type","category-data-in-repository","category-research-paper","cat-14-id","cat-110-id","cat-15-id"],"_links":{"self":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/posts\/3360","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/users\/106"}],"replies":[{"embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/comments?post=3360"}],"version-history":[{"count":0,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/posts\/3360\/revisions"}],"wp:attachment":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/media?parent=3360"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/categories?post=3360"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/tags?post=3360"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}