{"id":2962,"date":"2018-07-19T11:14:18","date_gmt":"2018-07-19T09:14:18","guid":{"rendered":"https:\/\/ist.blogs.inra.fr\/afs\/?p=2962"},"modified":"2018-07-19T11:14:18","modified_gmt":"2018-07-19T09:14:18","slug":"low-within-population-genetic-diversity-and-high-genetic-differentiation-among-populations-of-the-endangered-plant-tetracentron-sinense-oliver-revealed-by-inter-simple-sequence-repeat-analysis","status":"publish","type":"post","link":"https:\/\/ist.blogs.inrae.fr\/afs\/2018\/07\/19\/low-within-population-genetic-diversity-and-high-genetic-differentiation-among-populations-of-the-endangered-plant-tetracentron-sinense-oliver-revealed-by-inter-simple-sequence-repeat-analysis\/","title":{"rendered":"Low within-population genetic diversity and high genetic differentiation among populations of the endangered plant Tetracentron sinense Oliver revealed by inter-simple sequence repeat analysis"},"content":{"rendered":"<script type='text\/javascript' src='https:\/\/d1bxh8uas1mnw7.cloudfront.net\/assets\/embed.js'><\/script><p><em>Tetracentron sinense<\/em> Oliver, an endangered species from China, displays a low within-population genetic diversity and high genetic differentiation among populations, and the existing populations could be divided into three conservation and management units.<\/p>\n<p><strong>Context<\/strong> The endangered tree <em>Tetracentron sinense<\/em> Oliver has great value; however, little is known regarding the within-population genetic diversity and differentiation among <em>T. sinense<\/em> populations.<br \/>\n<strong>Aims<\/strong> We examined the genetic diversity and differentiation of <em>T. sinense<\/em> wild populations, and we tested the effect of small-size population on the level of genetic diversity within these populations.<br \/>\n<strong>Methods<\/strong> Using inter-simple sequence repeat (ISSR), we assessed the genetic variation and structure among 174 individuals from 26 natural populations of <em>T. sinense<\/em> sampled across its distribution range in China.<br \/>\n<strong>Results<\/strong> The ISSR primers yielded 180 amplified loci (123 were polymorphic). At the species level, the percentage of polymorphic loci (PPL), Nei\u2019s gene diversity (H), and Shannon\u2019s information index (I) were 68.3%, 0.196 and 0.300, respectively. The average population level PPL was 20.0%, and the Na, Ne, H, and I were 1.20, 1.13, 0.076, and 0.112, respectively. AMOVA revealed high genetic differentiation among populations (52.0% of total variance, P\u2009=\u20090.001), consistent with the gene differentiation coefficient (Gst\u2009=\u20090.607) and gene flow (Nm\u2009=\u20090.326). The 174 individuals of the 26 <em>T. sinense<\/em> populations clustered into three groups, and <em>T. sinense<\/em> geographic and genetic distance were significantly correlated.<br \/>\n<strong>Conclusions<\/strong> <em>T. sinense<\/em> exhibited intermediate within-species genetic diversity, indicating preserved evolutionary potential. The low within-population genetic diversity and high genetic differentiation among <em>T. sinense<\/em> populations may be one of important factors causing endangerment. Three conservation units were determined based on genetic difference and structure. Inter-population introduction of individuals within units via appropriate propagation and seedling management might be an effective strategy for increasing <em>T. sinense<\/em> within-population genetic diversity and population size.<\/p>\n<p>Keywords<br \/>\n<em>Tetracentron sinense<\/em> Oliver, Genetic variation, Genetic structure, Molecular marker, ISSR, Conservation strategy, China<\/p>\n<div class='altmetric-embed' data-badge-type='donut' data-doi='10.1007\/s13595-018-0752-4'  style='float: right; ' ><\/div>\n<p>Publication<br \/>\nLi, S., Gan, X., Han, H. et al. Annals of Forest Science (2018) 75: 74.<br \/>\n<a href=\"https:\/\/doi.org\/10.1007\/s13595-018-0752-4\">https:\/\/doi.org\/10.1007\/s13595-018-0752-4<\/a><\/p>\n<p>For the read-only version of the full text: <a href=\"https:\/\/rdcu.be\/3cxM\">https:\/\/rdcu.be\/3cxM<\/a><\/p>\n<p>Data availability<br \/>\nThe datasets generated and\/or analyzed during the current study are available in the GitHub repository, <a href=\"https:\/\/github.com\/bhgan\/Low-within-population-genetic-diversity-and-high-genetic-differentiation-among-populations-of-the-en\">https:\/\/github.com\/bhgan\/Low-within-population-genetic-diversity-and-high-genetic-differentiation-among-populations-of-the-en<\/a> [accessed 21 June 2018] or from the corresponding author on reasonable request.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tetracentron sinense Oliver, an endangered species from China, displays a low within-population genetic diversity and high genetic differentiation among populations, and the existing populations could be divided into three conservation and management units. Context The endangered tree Tetracentron sinense Oliver has great value; however, little is known regarding the within-population genetic diversity and differentiation among [&hellip;]<\/p>\n","protected":false},"author":94,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[14,110,15],"tags":[],"class_list":["post-2962","post","type-post","status-publish","format-standard","hentry","category-article-type","category-data-in-repository","category-research-paper","cat-14-id","cat-110-id","cat-15-id"],"_links":{"self":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/posts\/2962","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/users\/94"}],"replies":[{"embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/comments?post=2962"}],"version-history":[{"count":0,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/posts\/2962\/revisions"}],"wp:attachment":[{"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/media?parent=2962"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/categories?post=2962"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ist.blogs.inrae.fr\/afs\/wp-json\/wp\/v2\/tags?post=2962"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}